Neuropeptide FF (NPFF) receptors are a family of G protein-coupled receptors (GPCRs) comprised of two receptor subtypes: NPFF1 and NPFF2. NPFF1 is primarily found within the central nervous system, more specifically the limbic system and hypothalamus, while NPFF2 is primarily found within the spinal cord. NPFF plays a role in the regulation of food intake, temperature, hormonal modulation, and cardiovascular regulation. Recent studies have also shown a connection between the opioid system and NPFF, suggesting a role for NPFF receptors in drug related conditions. NPFF antagonists also have therapeutic applications in decreasing post-morphine withdrawal symptoms and preventing tolerance to analgesic treatments.