MULTISCREEN™ assay kits are developed for high sensitivity, specificity and compatibility for high throughput screening. They are amenable in highly miniaturized settings, in 384-well or higher density microplates. All of Multispan’s assay kits are NO-WASH HOMOGENEOUS assay.
MULTISCREEN™ assay kits are divided into 3 categories, addressing the 3 major and most studied GPCR cell signaling pathways. These 3 cell signaling pathways are measured by their respective intracellular second messengers: calcium, cAMP and beta-arrestin. Each of the 3 types of assay kits employs a different detection mechanism such as fluorescence, TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or chemiluminescence, designed to be best suited for the chemical and molecular nature of each specific second messenger being measured. The different readouts not only maximize the robustness and sensitivity of the assays, but also enable the possibility of multiplexing, ie, two assays in one well. Multiplex assays are in general much desired in HTS assays to save time and cost.