MULTISCREEN™ TR-FRET cAMP No Wash Assay Kits

A homogeneous TR-FRET assay for quantifying intracellular cAMP in 96-well, 384-well, and higher multiplexity. No wash steps and validated with MULTISCREEN™ stable cell lines and division-arrested cells for integrated screening workflows.
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Why MULTISCREEN™ TR-FRET cAMP?

Product Features
  • No-wash, homogeneous format
  • Minimal interference from colored/fluorescent compounds
  • Validated in 96-, 384-, and 1536-well HTS formats
  • Sub-30 minute hands-on time
  • Cross-validated with 500+ MULTISCREEN™ GPCR stable cell lines
  • Seamless integration with MULTISCREEN™ stable cell lines and division-arrested cells
Unit Size (384-Well) Catalog Number
500 tests
MSCM01-1
5000 tests
MSCM01-5
25000 tests
MSCM01-25
MULTISCREEN™ TR-FRET cAMP 1.0 No-Wash Assay Kits provide a homogeneous assay method for adenylyl cyclase activity detection in 96-well, 384-well, and higher multiplexity. The dual-label system uses an α-cAMP antibody labeled with MULTISCREEN™ Eu and cAMP labeled with MULTISCREEN™ 650.
Cyclic AMP Assay Kit Image
In the absence of free cAMP, MULTISCREEN™ 650-cAMP binds to MULTISCREEN™ Eu-α-cAMP-Ab, producing a strong TR-FRET emission at 655 nm. Free cAMP in the sample competes for binding to the antibody, reducing the TR-FRET signal. Because MULTISCREEN™ 650-cAMP has a fluorescence lifetime of only nanoseconds while MULTISCREEN™ Eu-α-cAMP-Ab has a much longer lifetime, the distinct fluorescence lifetimes enable precise determination of cAMP concentration, with the TR-FRET magnitude proportional to the cAMP in the sample.
Our Kits our compatible with most commercially available multimode microplate readers, and are validated with MULTISCREEN™ stable cell lines.
cAMP Dose-Response in MULTISCREEN™ β1-HEK293T stable cell line (C1437-1) measured with MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kit. Cells were seeded in a 384-well solid white plate, incubated with Isoproterenol and 1mM IBMX before being treated with MULTISCREEN™ Eu-α-cAMP-Ab and MULTISCREEN™ 650-cAMP, and measured by FlexStation 3.
Left Figure: cAMP Dose-Response in MULTISCREEN™ GLP-1 β-Arrestin2 HEK293T stable cell line and measured with MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kit.
Right Figure: Representative Z’-factor data from one data set conducted with MULTISCREEN™ GLP-1 β- Arrestin2 HEK293T stable cell line measured with MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kit on a BioCoat Poly-D-Lysine 384-well plate in the absence (red) or presence of agonist (blue).

Xelaglifam, a novel GPR40/FFAR1 agonist, exhibits enhanced β-arrestin recruitment and sustained glycemic control for type 2 diabetes

GPCR Target: MULTISCREENᵀᴹ TR-FRET cAMP 1.0 No Wash Assay Kit

Authors: Jongmin Yoon, et al.

Journal: Biomedicine & Pharmacotherapy, Volume 177, 2024, 117044, ISSN 0753-3322.

Year Published: 2024

New quinolone derivatives as neuropeptide S receptor antagonists: Design, synthesis, homology modeling, dynamic simulations and modulation of Gq/Gs signaling pathways.

GPCR Target: Calcium assay, cAMP assay

Authors: Batran RZ, Gugnani KS, Maher TJ, Khedr MA.

Company: Chemistry of Natural Compounds Department, Pharmaceutical and Drug Industries Research Division, National Research Centre,Dokki, Giza, Egypt; Department of Pharmaceutical Sciences, MCPHS University, Boston, MA, USA.; Pharmaceutical Chemistry Department, Faculty of Pharmacy, Helwan University, Ein Helwan, Cairo, Egypt

Journal: Bioorg Chem. 2021 Jun;111:104817

Year Published: 2021

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