Product Information
Catalog Number:
C1011a
Lot Number:
C1011-072424
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Cellbanker 2
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human CCR3 cDNA (GenBank Accession Number NM_001837) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin
Stability:
Stable for a minimum of 2 months in continuous culture
Data Sheet
Background: CCR3 (C-C chemokine receptor type 3 or eosinophil eotaxin receptor) is a receptor for a C-C type chemokine and binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. The receptor subsequently transduces a signal by increasing the intracellular calcium level. Similar to CCR2, it is another alternative coreceptor with CD4 for HIV-1 infection. CCR3 is expressed on eosinophils, basophils, mast cell subpopulations, activated Th2 cells, macrophages, and airway epithelial cells. Hence, CCR3 is closely associated with asthma and allergy and blockade of this receptor may have pronounced beneficial effects in these diseases.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
Erin et al. (2002) Eotaxin receptor (CCR3) antagonism in asthma and allergic disease. Curr Drug Targets Inflamm Allergy 1:201-214.
Gangur et al. (2003) CCR3 and CXCR3 as drug targets for allergy: principles and potential. Curr Drug Targets Inflamm Allergy 2:53-62.