Product Information
Catalog Number:
DCd1290a
Lot Number:
DCd1290a-112625
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium: Cellbanker 2
Host cell:
HEK293T
Transfection: Expression vector containing full-length dog GIPR cDNA (GenBank accession number XM_005616439.3) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Data Sheet
Background: GIP (gastric inhibitory polypeptide) is released from the gastrointestinal tract, stimulates insulin secretion from pancreatic beta-cells, and plays a crucial role in the regulation of insulin secretion. Its receptor GIPR is expressed in the pancreas, stomach, small intestine, adipose tissue, adrenal cortex, pituitary, heart, testis, endothelial cells, bone, trachea, spleen, thymus, lung, kidney, thyroid, and several regions in the CNS. GIPR may have therapeutic potential in the treatment of type 2 diabetes and obesity.
Application: Functional assays
Figure 1. Dose-dependent stimulation of intracellular cAMP level upon treatment with ligand, measured with MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
References:
Irwin et al. (2009) Therapeutic potential for GIP receptor agonists and antagonists. Best Pract Res Clin Endocrinol Metab 23:499-512.
Yamada et al. (1995) Human gastric ingibitory polypeptide receptor: cloning of the gene (GIPR) and cDNA. Genomics 29:773-776.