MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT GPR37L1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human GPR37L1 cDNA (GenBank Accession Number NM_004767) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after two months continuous growth
Background: GPR37L1 (G-protein coupled receptor 37 like 1) is also known as ETBR-LP-2 (Endothelin B receptor-like protein-2) and is an orphan receptor. The receptor is strongly expressed in the human central nervous system, e.g. in cerebellar Bergmann glia, cerebral cortex, and internal capsule fibers. The fragment of this receptor is detectable in human cerebrospinal fluid.
Application: Functional analysis
Figure 1. Dose-dependent inhibition of forskolinstimulated intracellular cAMP level upon treatment with ligand, measured with cAMP HiRange kit (Cisbio 62AM6PEC). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Valdenaire et al. (1998) A new family of orphan G protein-coupled receptors predominantly expressed in the brain. FEBS Lett 424:193-196.
Stark et al. (2001) Peptide repertoire of human cerebrospinal fluid: novel proteolytic fragments of neuroendocrine proteins. J Chromatogr B Biomed Sci Appl 754:357- 367.