RAT RECOMBINANT MU OPIOID RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length rat Oprm1 opioid receptor cDNA (GenBank Accession Number L13069)
Liquid nitrogen upon receiving
Propagation Medium: Alpha-MEM, 10% FBS, 800 µg/mL G418
Background: Mu opioid receptor (MOR) is a receptor for beta-endorphin, inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. MOR signaling and regulation are strongly agonist-dependent. MOR mediates positive reinforcement following direct (morphine) or indirect (alcohol, cannabinoids, nicotine) activation. MOR plays a genetic role in the control of gut inflammation. MOR-deficient mice are highly susceptible to colon inflammation, with a 50% mortality rate occurring 3 days after administration of TNBS that induces inflammation. MOR agonists regulate cytokine production and T cell proliferation and might be new therapeutic molecules in inflammatory bowel disease.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, monitored with FlexStation. Cells were transiently transfected with Gαqi5. Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with cAMP HiRange kit (Cisbio 62AM6PEC).
Chen et al. (1993) Molecular cloning and functional expression of a mu-opioid receptor from rat brain. Mol Pharmacol 44:8-12.
Contet et al. (2004) Mu opioid receptor: a gateway to drug addiction. Curr Opin Neurobiol 14:370-378.
Philippe et al. (2003) Anti-inflammatory properties of the mu opioid receptor support its use in the treatment of colon inflammation. J Clin Invest 111:1329-1338.