Product Information
Catalog Number:
C1098
Lot Number:
C1098-013006
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Sigma Freezing Medium (C-6164)
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human GPR37L1 cDNA (GenBank Accession Number NM_004767) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stability:
Stable after two months continuous growth
Data Sheet
Background: GPR37L1 (G-protein coupled receptor 37 like 1) is also known as ETBR-LP-2 (Endothelin B receptor-like protein-2) and is an orphan receptor. The receptor is strongly expressed in the human central nervous system, e.g. in cerebellar Bergmann glia, cerebral cortex, and internal capsule fibers. The fragment of this receptor is detectable in human cerebrospinal fluid.
Application: Functional analysis
Figure 1. Dose-dependent inhibition of forskolin stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
Valdenaire et al. (1998) A new family of orphan G protein-coupled receptors predominantly expressed in the brain. FEBS Lett 424:193-196.
Stark et al. (2001) Peptide repertoire of human cerebrospinal fluid: novel proteolytic fragments of neuroendocrine proteins. J Chromatogr B Biomed Sci Appl 754:357- 367.