HUMAN RECOMBINANT GHRELIN RECEPTOR
MULTISCREEN™ MEMBRANE PREPARATION
1 vial (7.1mg/ml, 50ul)
Full-length mouse Ghsr cDNA (GenBank Accession Number NM_198407.1) with FLAG-tag sequence at the N-terminus
Background: The ghrelin receptor is the target of growth hormone secretagogues, a class of synthetic peptide and non-peptide compounds that stimulate growth hormone (GH) release from the anterior pituitary. Ghrelin, the endogenous ligand for the ghrelin receptor, is predominantly secreted from X/A-like cells within the gastric mucosa and may be the source of the majority of circulating plasma ghrelin. Ghrelin stimulates gastric acid secretion and motility, and may have significant effects on appetite and energy. It is not only important for the acute regulation of food intake but also plays an important role in the regulation of long term energy homoeostasis. Ghrelin has a number of actions in cardiovascular system, consistent with the localization of receptors to cardiovascular tissue.
Application: Radioligand Binding Assay
Figure 1. Saturation binding assay curve: Specific binding obtained with ghrelin membrane with [125I] Ghrelin. Scatchard plot showed that there is one binding site. Figure 2. Competition binding assay curve: Dose-dependent competition of agonists for [125I] Ghrelin 0.5nM. Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Howard et al. (1996) A receptor in pituitary and hypothalamus that functions in growth hormone release. Science 273:974-977.
Kojima and Kangawa (2005) Ghrelin: structure and function. Physiol Rev 85:495-522.
van der Lely et al. (2004) Biological, physiological, pathophysiological, and pharmacological aspects of ghrelin. Endocr Rev 25:426-457.