HUMAN RECOMBINANT XCR1 RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human XCR1 cDNA (GenBank accession number NM_005283.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: Chemokines and their receptors play a vital role in the regulation of responses in the immune system and in the recruitment of certain lymphocytes. The human chemokine receptor XCR1 is a G-protein-coupled receptor with 333 amino acids. Specifically, XCR1 has been associated with the induction of chemotaxis and has been identified in neutrophils, t-lymphocytes, b-lymphocytes through RT-PCR.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Huang et al. (2001) Neutrophils and B cells express XCR1 receptor and chemotactically respond to lymphotactin. Biochem Biophys Res Commun 281:378- 382.
Yoshida et al. (1998) Identification of single C motif-1/lymphotactin receptor XCR1. J Biol Chem 273:16551-16554.