HUMAN RECOMBINANT TRKA RECEPTOR

MULTISCREEN™ STABLE CELL LINES

Product Information

Catalog Number:
CK1001-1b

Lot Number:
CK1001-1b-102615

Quantity:
1 vial (2 x 10⁶) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human FGFR3 cDNA (GenBank Accession Number NM_001012331) and vector pGL4.30[luc2P/NFAT-RE/Hygro] (GenBank Accession Number DQ904462)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 400 µg/mL G418; 250 µg/mL hygromycin

Stability:
In progress

Data Sheet

Background: Neurotrophic tyrosine kinase receptor type 1 (also called NTRK1; MTC; p140-TrkA; TRK; Trk-A; TRK1; TRKA) is a member of the neurotrophic tyrosine kinase receptor family. NTRK1 is a membrane bound receptor that phosphorylates itself and members of the MAPK pathway upon neurotrophin binding. Genetic variations in NTRK1 have been linked to anhidrosis, congenital insensitivity to pain, cancer, and mental retardation.

Application: Functional assays

Figure 1. Dose-dependent calcium flux upon treatment with ligand, monitored with FLIPR.

References:

Bradshaw, R.A. et al. “Receptor Tyrosine Kinase Signaling Mechanisms: Devolving TrkA Responses with Phosphoproteomics.” Advances in biological regulation 53.1 (2013): 87–96. PMC. Web. 4 Feb. 2015.

Mardy, S et al. “Congenital Insensitivity to Pain with Anhidrosis: Novel Mutations in the TRKA (NTRK1) Gene Encoding a High-Affinity Receptor for Nerve Growth Factor.” American Journal of Human Genetics 64.6 (1999): 1570–1579. Print.

Marlin, M. Caleb, and Guangpu Li. “Biogenesis and Function of the NGF/TrkA Signaling Endosome.” International review of cell and molecular biology 314 (2015): 239–257. PMC. Web. 4 Feb. 2015.