HUMAN RECOMBINANT TRKA RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human FGFR3 cDNA (GenBank Accession Number NM_001012331) and vector pGL4.30[luc2P/NFAT-RE/Hygro] (GenBank Accession Number DQ904462)
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 400 µg/mL G418; 250 µg/mL hygromycin
Background: Neurotrophic tyrosine kinase receptor type 1 (also called NTRK1; MTC; p140-TrkA; TRK; Trk-A; TRK1; TRKA) is a member of the neurotrophic tyrosine kinase receptor family. NTRK1 is a membrane bound receptor that phosphorylates itself and members of the MAPK pathway upon neurotrophin binding. Genetic variations in NTRK1 have been linked to anhidrosis, congenital insensitivity to pain, cancer, and mental retardation.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, monitored with FLIPR. Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-NTRK1 antibody. Thin line: parental cells; thick line: receptor- expressing cells.
Bradshaw, R.A. et al. “Receptor Tyrosine Kinase Signaling Mechanisms: Devolving TrkA Responses with Phosphoproteomics.” Advances in biological regulation 53.1 (2013): 87–96. PMC. Web. 4 Feb. 2015.