HUMAN RECOMBINANT SST5 RECEPTOR

MULTISCREEN™ STABLE CELL LINES

Product Information

Catalog Number:
C1349-1

Lot Number:
C1349-1-030609

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human SSTR5 cDNA (GenBank Accession Number NM_001053) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin

Stability:
Stable after minimum two months continuous growth

Data Sheet

Background: Somatostatin receptors (SSTRs) are activated by somatostatin secreted from nerve and endocrine cells. SSTRs are expressed in a tissue-specific manner and involved in the regulation of secretion of insulin, glucagon and growth hormone as well as cell growth induced by neuronal excitation in both the central and peripheral nervous systems. Aberrant expression of somatostatin receptors is known in a large number of human tumors. SSTR5 is expressed in adult pituitary gland, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. SSTR5 is a candidate gene for bipolar affective disorder as well as for other neuropsychiatric disorders. Expression of SSTR5 may be important in the growth inhibitory effect of somatostatin in human pancreatic cancer.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose- dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Ardjomand et al. (2003) Expression of somatostatin receptors in uveal melanomas. Invest Ophthal Vis Sci 44:980-987.

Ballare et al. (2001) Mutation of somatostatin receptor type 5 in an acromegalic patient resistant to somatostatin analog treatment. J Clin Endocr Metab 86:3809- 3814.

FOR RESEARCH USE ONLY.
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