HUMAN RECOMBINANT RXFP1 RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human RXFP1 cDNA (GenBank accession number: NM_021634) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: The relaxin family peptide receptor RXFP1, also known as LGR7 or RX1, is a 757-amino acid 7 transmembrane protein. RT-PCR detected RXFP1 expression in various organs of the reproductive system along with the brain, heart, and lungs. RXFP1 is known to be important to the connective tissue cells of the breast and play a central role in endometrial deciduation.
Application: Functional assays
Figure 1. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Ivell et al. (2003) Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates. Reprod Biol Endocrinol 1:114-114.
Hsu et al. (2002) Activation of orphan receptors by the hormone relaxin. Science 295:671-674.