Product Information
Catalog Number:
MC1160-3
Lot Number:
MC1160-3-011426
Quantity:
5 mg per vial
Protein concentration: 3.6 mg/mL
Packaging Buffer: 20mM Gly-Gly, 1 mM MgCl2, 25mM Sucrose (pH 7.2)
Host cell:
1321N1
Transfection:
Full-length human P2Y1 cDNA (GenBank Accession Number NM_002563)
Recommended Storage:
Liquid nitrogen upon receiving
Data Sheet
Background: P2Y1 is a receptor for ATP and ADP. P2Y1 contributes to platelet shape change. A number of P2Y1 receptor-specific antagonists, such as, MRS-2179, A3P5PS, A3P5P and A2P5P have been discovered. They inhibit calcium ion mobilization and shape change in platelets. P2Y1-deficient mice and mice treated with the P2Y1 antagonist MRS2179 displayed significantly less arterial thrombosis than their respective controls. Combination of P2Y1 deficiency with P2Y12 inhibition led to a significant additive effect.
Application: Functional assays
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
Baurand and Gachet (2003) The P2Y(1) receptor as a target for new antithrombotic drugs: a review of the P2Y(1) antagonist MRS-2179. Cardiovasc Drug Rev 21:67-76.
Jin et al. (1998) Molecular basis for ADP-induced platelet activation. II. The P2Y1 receptor mediates ADP-induced intracellular calcium mobilization and shape change in platelets. J Biol Chem 273:2030-2034.
Lenain et al. (2003) Inhibition of localized thrombosis in P2Y1-deficient mice and rodents treated with MRS2179, a P2Y1 receptor antagonist. J Thromb Haemost 1:1144-1149.