HUMAN RECOMBINANT NPY1 RECEPTOR
MULTISCREEN™ DIVISION ARRESTED CELL LINE
1 vial (4x 106) frozen cells
Freeze Medium: Cellbanker 2 (Amsbio)
Expression vector containing full-length human NPY1R cDNA (GenBank Accession Number NM_000909) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM-F12, 10% FBS
Background: NPY1R (Neuropeptide Y receptor type 1) is a receptor for neuropeptide Y that is the most abundant peptide present in the mammalian central and peripheral nervous system. Neuropeptide Y exhibits a variety of potent central and peripheral effects including those on feeding, memory, blood pressure, cardiac contractility and intestinal secretions. Neuropeptide Y effects are mediated by several different receptor subtypes, Y- 1, Y-1-like, Y-2, Y-3, Y-4 and Y-5. NPY1R is correlated with food feeding and subnanomolar antagonists of NPY1R have been discovered as potential anti- obesity agents. NPY1R is also involved in cardiovascular actions of exogenous neuropeptide Y and plays a role in maintaining basal blood pressure and heart rate in ischaemic heart failure.
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MULTISCREENTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
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Zhao et al. (1999) Renal and cardiovascular role of the neuropeptide Y Y1 receptor in ischaemic heart failure rats. J Pharm Pharmacol 51:1257-1265.