HUMAN RECOMBINANT NPBW1 RECEPTOR
MULTISCREEN™ DIVISION-ARRESTED CELL LINE
1 vial (4 x 106) frozen cells
Cellbanker 2 (Amsbio)
Full-length human NPBWR1 cDNA (GenBank Accession Number NM_005285)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Background: Neuropeptide B/neuropeptide W receptor 1 (NPBW1 or GPR7) is a receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals. Targeted disruption of NPBW1 in mice has confirmed that this receptor plays a role in maintaining long-term energy homeostasis. NPBW1 lacking male mice show moderately severe, late-onset obesity that is a result of both hyperphagia and decreased energy expenditure with reduced locomotor activity.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MULTISCREEN™ Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Levine et al. (2005) Injection of neuropeptide W into paraventricular nucleus of hypothalamus increases food intake. Am J Physiol Regul Integr Comp Physiol 288:R1727-R1732.
Ishii et al. (2003) Targeted disruption of GPR7, the endogenous receptor for neuropeptides B and W, leads to metabolic defects and adult-onset obesity. Proc Natl Acad Sci USA 100: 0540-10545.