HUMAN RECOMBINANT NK2 RECEPTOR
MULTISCREEN™ DIVISION-ARRESTED CELL LINE
1 vial (4 x 106) frozen cells
Cellbanker 2 (Amsbio)
Expression vector containing full-length human TACR2 cDNA (GenBank Accession Number NM_001057) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Background: The tachykinin receptor 2 (TACR2 or NK2) is a receptor for the tachykinin family of peptide neurotransmitters. NK2 receptor selectively binds neurokinin A (substance K), which is predominating in human and animal airways. It has been shown to regulate circular muscle contraction. NK2 can be found in tracheal and gastric tissues.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MULTISCREEN™ Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Sasai and Nakanishi (1989) Molecular characterization of rat substance K receptor and its mRNAs. Biochem Biophys Res Commun 165:695-702.
Morten et al. (1991) Human NK-2 receptor gene maps to chromosome region 10q11- 21. Hum Genet 88:200-203.