Product Information
Catalog Number:
C1304
Lot Number:
C1304-073005
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Sigma Freezing Medium (C-6164)
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human TACR2 cDNA (GenBank Accession Number NM_001057) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stability:
Stable after minimum of two months continuous growth
Data Sheet
Background: The tachykinin receptor 2 (TACR2 or NK2) is a receptor for the tachykinin family of peptide neurotransmitters. NK2 receptor selectively binds neurokinin A (substance K), which is predominating in human and animal airways. It has been shown to regulate circular muscle contraction. NK2 can be found in tracheal and gastric tissues.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
Sasai and Nakanishi (1989) Molecular characterization of rat substance K receptor and its mRNAs. Biochem Biophys Res Commun 165:695-702.
Morten et al. (1991) Human NK-2 receptor gene maps to chromosome region 10q11- 21. Hum Genet 88:200-203.