HUMAN RECOMBINANT NK1 RECEPTOR
MULTISCREEN™ DIVISION-ARRESTED CELL LINE
1 vial (4 x 106) frozen cells
Cellbanker 2 (Amsbio)
Expression vector containing full-length human TACR1 cDNA (GenBank Accession Number NM_001058.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Background: NK1 (or tachykinin receptor TACR1) is a selective receptor for substance P with relatively higher affinity for neurokinin A compared to neurokinin B (100- and 500-fold lower). Neurons in lamina I of the spinal dorsal horn express the NK1 receptor for substance P and mediate hyperalgesia, an enhanced sensitivity to painful stimuli. Although substance P does not mediate the signaling of acute pain or hyperalgesia, it is essential for the full development of stress-induced analgesia and for an aggressive response to territorial challenge. It is also suggested that substance P is important for orchestrating the response of the animal to major stressors such as pain, injury or invasion of territory. Modulation of substance P activity offers a radical new approach to the management of depression, anxiety and stress. The substance P receptor is highly expressed in areas of the brain that are implicated in these behaviors.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MULTISCREEN™ Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
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Quartara and Maggi (1997) The tachykinin NK1 receptor. Part I: ligands and mechanisms of cellular activation. Neuropeptides 31:537-563.
Quartara and Maggi (1998) The tachykinin NK1 receptor. Part II: distribution and pathophysiological roles. Neuropeptides 32:1-49.