MULTISCREEN™ STABLE CELL LINES
Product Information
Catalog Number:
C1527
Lot Number:
C1527-C106-012419
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Cellbanker 2
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human TMEM175 cDNA (GenBank Accession Number NM_032326.3) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stability:
In progress
Data Sheet

Figure 1. Dose-dependent potassium channel activity upon stimulation of Tl+, monitored on FLIPR Figure 2 Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
Chunlei Cang et al. “TMEM175 Is an Organelle K(+) Channel Regulating Lysosomal
Function.”Cell 162: 1101–1112. 27 Aug. 2015.