MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT D3 RECEPTOR
1 vial (2 x 106) frozen cells
Cell Banker 2 (Amsbio 11891)
Expression vector containing full-length human DRD3 cDNA (GenBank accession number NM_000796.3 with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin, 250 μg/mL hygromycin
Background: The human dopamine receptor DRD3 (D3) is a G protein-coupled receptor for dopamine. D3 receptor belongs to the D2-like family of dopamine receptors which are widely expressed in the central nervous system. D3 is involved in movement coordination, cognition, emotion, affects memory and the regulation of prolactin secretion by the pituitary. Alterations in D3 pathways can cause pathogenesis of neurological, neuropsychiatric and hormonal disorders, including Parkinson’s Disease, schizophrenia and hyperprolactinemia.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Missale, C., Nash, S. R., Robinson, S. W., Jaber, M., & Caron, M. G. (1998). Dopamine receptors: from structure to function. Physiological reviews, 78(1), 189- 225.
Taylor, M., Grundt, P., Griffin, S. A., Newman, A. H., & Luedtke, R. R. (2010). Dopamine D3 receptor selective ligands with varying intrinsic efficacies at adenylyl cyclase inhibition and mitogenic signaling pathways. Synapse, 64(3), 251-266.