MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT ZNT8 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human ZnT8 cDNA (GenBank Accession Number NM_173851.2)
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 400 µg/mL G418, Non- Essential Amino Acids
Background: Zinc transporter ZnT-8 is highly expressed in pancreatic β-cells and is a key protein for both zinc accumulation and regulation of insulin secretion. Studies have demonstrated that mice lacking ZnT-8 globally were more susceptible to diet induced obesity. It has been suggested that ZnT-8 contributes to the risk of developing type 2 diabetes through β-cell- and non-β-cell-specific effects.
Application: Functional assays
Figure 1. Dose-dependent zinc transport upon treatment with ligand, monitored with Flexstation.