MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT ZNT3 RECEPTOR

Product Information

Catalog Number:
C2001-7

Lot Number:
C2001-7-050415

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
Hela

Transfection:
Expression vector containing full-length human ZnT3 cDNA (GenBank Accession Number NM_003459.4)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 400 µg/mL G418, Non- Essential Amino Acids

Stability:
In progress

Data Sheet

Background: Zinc transporter-3 (ZnT-3) is expressed in regions of the brain that are rich in histochemically reactive zinc, including entorhinal cortex, amygdala, and hippocampus. ZnT-3 protein is most abundant in the zinc-enriched mossy fibers that project from the dentate granule cells to hilar and CA3 pyramidal neurons.

Application: Functional assays

Figure 1. Dose-dependent zinc transport upon treatment with ligand, monitored with Flexstation.

References:

Stefano L. Sensi et al. “The Neurophysiology and Pathology of Brain Zinc.”J Neurosci. 2011 November 9; 31(45): 16076–16085. doi:10.1523/JNEUROSCI.3454-11.2011

Damitha De Mel and Cenk Suphioglu. “Fishy Business: Effect of Omega-3 Fatty Acids on Zinc Transporters and Free Zinc Availability in Human Neuronal Cells”. Nutrients 2014, 6, 3245-3258; doi:10.3390/nu6083245
 
Isamu Aiba1, et al. “Intracellular Dialysis Disrupts Zn2+ Dynamics and Enables Selective Detection of Zn2+ Influx in Brain Slice Preparations.” J Neurochem. 2013
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