MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT ZNT3 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human ZnT3 cDNA (GenBank Accession Number NM_003459.4)
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 400 µg/mL G418, Non- Essential Amino Acids
Background: Zinc transporter-3 (ZnT-3) is expressed in regions of the brain that are rich in histochemically reactive zinc, including entorhinal cortex, amygdala, and hippocampus. ZnT-3 protein is most abundant in the zinc-enriched mossy fibers that project from the dentate granule cells to hilar and CA3 pyramidal neurons.
Application: Functional assays
Figure 1. Dose-dependent zinc transport upon treatment with ligand, monitored with Flexstation.
Stefano L. Sensi et al. “The Neurophysiology and Pathology of Brain Zinc.”J Neurosci. 2011 November 9; 31(45): 16076–16085. doi:10.1523/JNEUROSCI.3454-11.2011