HUMAN RECOMBINANT TMEM175 RECEPTOR

MULTISCREEN™ STABLE CELL LINES

Product Information

Catalog Number:
C1527

Lot Number:
C1527-C106-012419

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cellbanker 2

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human TMEM175 cDNA (GenBank Accession Number NM_032326.3) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
In progress

Data Sheet

Background: Transmembrane Protein 175 (TMEM175) is an organelle specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. TMEM175 is reported to regulate lumenal pH stability and is required for autophagosome-lysosome fusion. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability Application: Functional assays

Figure 1. Dose-dependent potassium channel activity upon stimulation of Tl+, monitored on FLIPR Figure 2 Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Chunlei Cang et al. “TMEM175 Is an Organelle K(+) Channel Regulating Lysosomal
Function.”Cell 162: 1101–1112. 27 Aug. 2015.

FOR RESEARCH USE ONLY.
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