MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT CGRP RECEPTOR

Product Information

Catalog Number:
C1515-1

Lot Number:
C1515-1- 081417

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cell Banker 2 (Amsbio 11891)

Host cell:
CHO-K1

Transfection:
Full-length Human CALCRL cDNA (GenBank Accession Number ( NM_005795) with FLAG-tag sequence at the N-terminus and Full- length Human receptor activity modifying protein 1 (RAMP1) cDNA (GenBank Accession Number BC000548) with myc-tag at the C- terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DME/F12, 10% FBS, 10 µg/mL puromycin, 250µg/mL hygromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: CALCRL (calcitonin receptor-like receptor) is a calcitonin family receptor. The function and pharmacology of this receptor is modified in the presence of receptor activity-modifying proteins (RAMPs), which are single Transmembrane domain proteins of about 160 amino acids, having three isoforms: RAMP1, RAMP2 and RAMP3. CALCR can function either as a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin (AM) receptor, depending on which members of RAMPs, are co-expressed. The CALCRL/RAMP1 complex has been known as the CGRP receptor and has a very high affinity for CGRP.

Application: Functional assays

Figure 1. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Morfis et al. (2008) Receptor Activity-Modifying Proteins Differentially Modulate the G Protein-coupling Efficiency of Amylin Receptors. Endocrinology: 149(11):5423–5431.

Hay et al. (2005) Pharmacological Discrimination of Calcitonin Receptor: Receptor Activity-Modifying Protein Complexes. Mol Pharmacol 67:1655–1665.

Gorn et al. (1992) Cloning, characterization, and expression of a human calcitonin receptor from an ovarian carcinoma cell line. J Clin Invest 90:1726-1735.

RJ Bailey et al.(2011). Pharmacological characterization of rat amylin receptors: implications for the identification of amylin receptor subtypes. BJP 166:151–167

FOR RESEARCH USE ONLY.
All rights reserved. No part of this document may be reproduced in any form without prior permission in writing.

26219 Eden Landing Road Hayward, CA 94545 | (510) 887-0817 | info@multispaninc.com