MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT α2C RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human ADRA2C cDNA (GenBank Accession Number AY455666) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin
Stable in culture for minimum of two months
Background: The human adrenergic α2c receptor is a 458-amino acid, 7- transmembrane protein. It belongs to the group of nine adrenoceptors that mediate the biological actions of the endogenous catecholamines adrenaline and noradrenaline. All three alpha2-receptor subtypes may operate as presynaptic inhibitory feedback receptors to control the release of neurotransmitters. The α2c receptor is of particular importance as it appears to regulate venous vasoconstriction.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Gyires et al. (2009) alpha(2)-Adrenoceptor subtypes-mediated physiological, pharmacological actions. Neurochem Int 55:447-453.
Knaus et al (2007) Alpha2-adrenoceptor subtypes–unexpected functions for receptors and ligands derived from gene-targeted mouse models. Neurochem Int 51:277-281.