MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT A1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human ADORA1 cDNA (GenBank Accession Number NM_000674) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable for a minimum of 2 months in continuous culture
Background: Adenosine A1 receptor (ADORA1 or RDC7) is a G protein- coupled receptor for adenosine. RT-PCR detected A1 expression in the brain, colon, jejunum, ileum and kidney. A1 functions in sleep-wake regulation, the fertilization process, and modulates coronary blood flow.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Ethier and Madison (2006) Adenosine A1 receptors mediate mobilization of calcium in human bronchial smooth muscle cells. Am J Respir Cell Mol Biol 35:496-502.
Zhai, W., Chen, D., Shen, H., Chen, Z., Li, H., Yu, Z., & Chen, G. (2016). A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Molecular Brain, 9, 66. http://doi.org/10.1186/s13041-016-0247-x