MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CALCRL RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length Human CALCRL cDNA (GenBank Accession Number NM_005795) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: CALCRL (Calcitonin gene-related peptide type 1 receptor) is a receptor for calcitonin-gene-related peptide (CGRP) in the presence of the receptor activity modifying protein 1 (RAMP1) and receptor for adrenomedullin in the presence of RAMP2 or RAMP3. CALCRL is predominantly expressed in the lung and heart. The receptor is considered as a potential target for anti-migraine drugs.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent stimulation of intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.
Aiyar et al. (1996) A cDNA encoding the calcitonin gene-related peptide type 1 receptor. J Biol Chem 271:11325-11329.
Mitsikostas and Sanchez del Rio (2001) Receptor systems mediating c-fos expression within trigeminal nucleus caudalis in animal models of migraine. Brain Res Brain Res Rev 35:20-35.