MULTISCREEN™ STABLE CELL LINES

MOUSE RECOMBINANT GPR119 RECEPTOR

Product Information

Catalog Number:
Cm1298-1

Lot Number:
Cm1298-1-050109

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length mouse GPR119 cDNA (GenBank Accession Number: BC115558) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM-F12, 10% FBS, 10 μg/mL puromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: GPR119 is a G protein-coupled receptor for the endogenous lipid signaling agent oleoylethanolamide (OEA). GPR119 is predominantly expressed in the pancreas (beta-cells) and gastrointestinal tract (enteroendocrine cells). In vitro studies have indicated a role for GPR119 in the modulation of insulin release by pancreatic beta-cells and of GLP-1 secretion by gut enteroendocrine cells.

Application: Functional assays

Figure Legend: Dose-dependent accumulation of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).

References:

Hilary et al. (2006) Deorphanization of a G protein-coupled receptor for oleoylethanolamide and its use in the discovery of smallmolecule hypophagic agents. Cell Metabolism 3:167-175.

Overton et al. (2007) GPR 119, a novel G protein-coupled receptor target for the treatment of type 2 diabetes and obesity. Br J Pharmacol 153 suppl 1:S76-S81.

FOR RESEARCH USE ONLY.
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