MULTISCREEN™ STABLE CELL LINES

FERRET RECOMBINANT GIP RECEPTOR

Product Information

Catalog Number:
Cf1290

Lot Number:
Cf1290-080818

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cellbanker 2 (Amsbio 11891)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length ferret GIPR cDNA (GenBank accession number M3Y308) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable for a minimum of 2 months in continuous culture

Data Sheet

Background: GIP (gastric inhibitory polypeptide) is released from the gastrointestinal tract, stimulates insulin secretion from pancreatic beta-cells, and plays a crucial role in the regulation of insulin secretion. Its receptor GIPR is expressed in the pancreas, stomach, small intestine, adipose tissue, adrenal cortex, pituitary, heart, testis, endothelial cells, bone, trachea, spleen, thymus, lung, kidney, thyroid, and several regions in the CNS. GIPR may have therapeutic potential in the treatment of type 2 diabetes and obesity.

Application: Functional assays

Figure 1. Dose-dependent stimulation of intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Irwin et al. (2009) Therapeutic potential for GIP receptor agonists and antagonists. Best Pract Res Clin Endocrinol Metab 23:499-512.

Yamada et al. (1995) Human gastric ingibitory polypeptide receptor: cloning of the gene (GIPR) and cDNA. Genomics 29:773-776.

FOR RESEARCH USE ONLY.
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