MULTISCREEN™ STABLE CELL LINES
CYNOMOLGUS RECOMBINANT P2Y14 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length Cynomolgus P2Y14 cDNA (GenBank Accession Number XM_005546114) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin, 250 µg/mL hygromycin
Stable in culture for a minimum of 2 months
Background: P2Y14 (GPR105) is a purinergic G protein-coupled receptor that specifically responds to UDP-glucose and related sugar-nucleotides. This receptor has important structural similarities to known members of the P2Y receptor family but also shows a distinctly different pharmacological profile, as the receptor does not respond to ATP, ADP, UTP, UDP, other nucleotides, dinucleotides, and nucleosides. Widespread distribution of P2Y14 has been observed in humans, with highest expression in placenta, adipose tissue, stomach and intestine, and moderate levels in the brain, spleen, lung and heart. In transfected cells, the P2Y14 receptor reportedly couples to pertussis toxin-sensitive Gαi/o proteins. However, the transduction pathway(s) used by this receptor in native systems still remains to be defined.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Abbracchio et al. (2003) Characterization of the UDP-glucose receptor (re-named here the P2Y14 receptor) adds diversity to the P2Y receptor family. Trends Pharmacol Sci 24:52-55.
Lee et al. (2003) P2Y-like receptor, GPR105 (P2Y14), identifies and mediates chemotaxis of bone-marrow hematopoietic stem cells. Genes Dev 17:1592-1604.