MULTISCREEN™ STABLE CELL LINES
MOUSE RECOMBINANT GPR120 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full length mouse GPR120 cDNA (GenBank accession number BC053698) with FLAG tag sequence at N-terminus and chimeric G protein Gαqi5
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin, 250 μg/mL hygromycin
Stable after two month continuous growth
Background: GPR120 is a G protein-coupled receptor for the long-chain free fatty acids. GPR120 mediated calcium mobilization, Erk1/Erk2 activation and GLP1 secretion. Unsaturated long-chain FFAs had a dose-dependent stimulatory effect, and α-linolenic acid was the most potent. GPR120 and GLP1 colocalized in human colonic intraepithelial neuroendocrine cells, and GPR120 may mediate dietary FFA- stimulated GLP1 secretion.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor Expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Fredriksson et al. (2003) Seven evolutionarily conserved human rhodopsin G protein- coupled receptors lacking close relatives. FEBS Lett 554:381-388.
Hirasawa et al. (2005) Free fatty acids regulate gut incretin glucagon-like peptide-1 secretion through GPR120. Nature Med 11:90-94.