MULTISCREEN™ STABLE CELL LINES

MOUSE RECOMBINANT GPR120 RECEPTOR

Product Information

Catalog Number:
CGm1294

Lot Number:
CGm1294-031809

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T Gαqi5

Transfection:
Full length mouse GPR120 cDNA (GenBank accession number BC053698) with FLAG tag sequence at N-terminus and chimeric G protein Gαqi5

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin, 250 μg/mL hygromycin

Stability:
Stable after two month continuous growth

Data Sheet

Background: GPR120 is a G protein-coupled receptor for the long-chain free fatty acids. GPR120 mediated calcium mobilization, Erk1/Erk2 activation and GLP1 secretion. Unsaturated long-chain FFAs had a dose-dependent stimulatory effect, and α-linolenic acid was the most potent. GPR120 and GLP1 colocalized in human colonic intraepithelial neuroendocrine cells, and GPR120 may mediate dietary FFA- stimulated GLP1 secretion.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor Expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Fredriksson et al. (2003) Seven evolutionarily conserved human rhodopsin G protein- coupled receptors lacking close relatives. FEBS Lett 554:381-388.

Hirasawa et al. (2005) Free fatty acids regulate gut incretin glucagon-like peptide-1 secretion through GPR120. Nature Med 11:90-94.

FOR RESEARCH USE ONLY.
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