MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT GPR84 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human GPR84 cDNA (GenBank accession Number NM_020370) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DME/F12, 10% FBS, 10 µg/mL puromycin, 250 µg/mL hygromycin
Stable in culture for minimum of two months
Background: Human GPR84 is a G protein-coupled receptor with 396 amino acids. The hgpr84 transcript was found in brain, heart, muscle, colon, thymus, spleen, kidney, liver, intestine, placenta, lung and leukocytes. A study in GPR84-deficient mice revealed a novel role for GPR84 in regulating early IL-4 gene expression in activated T cells in response to CD3 crosslinking.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Wittenberger et al. (2001) An expressed sequence tag (EST) data mining strategy succeeding in the discovery of new G-protein coupled receptors. J Mol Biol 307:799- 813.
Venkataraman and Kuo (2005) The G-protein coupled receptor, GPR84 regulates IL-4 production by T lymphocytes in response to CD3 crosslinking. Immunol Lett 101:144- 153.