MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT GPR34 RECEPTOR
1 vial (2 x 106) frozen cells
Cellbanker 2 (Amsbio 11891)
Expression vector containing full-length Human GPR34 cDNA (GenBank Accession Number: NM_005300) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin, 250 µg/mL hygromycin
Background: GPR34 is an orphan G protein coupled receptor, belongs to P2Y receptor family. It is a potent mediator of lysophospholipid and expressed in various tissues including stomach Mast cells, placenta, spleen, thymus, ovary, Heart, brain etc. GPR34 receptor is responsible for enhancing the antigen induced degranulation of mast cells.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
K. Makide and J. Aoki (2013) GPR34 as a lysophosphatidylserine receptor. J. Biochem. 2013;153 (4):327–329.
H. Kitamura et al. (2012) GPR34 is a receptor for lysophosphatidylserine with a fatty acid at the sn-2 position J. Biochem. 2012;151 (5):511–518
T. Sugo et al.(2006) Identification of a lysophosphatidylserine receptor on mast cells. Biochemical and Biophysical Research Communications 341 (2006) 1078–1087