MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CXCR2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CXCR2 cDNA (GenBank Accession Number NM_001557) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin, 250 µg/mL hygromycin
Stable in culture for minimum of two months
Background: CXCR2 (high affinity interleukin-8 receptor B, IL8RB) is a receptor to interleukin-8, which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This receptor also binds to GRO/MGSA and NAP-2 with high affinity. Some highly potent small molecule antagonists of CXCR2 showed success in blocking in vivo trafficking of neutrophils, suggesting that antagonism of IL-8 at the receptor level is a viable therapeutic strategy in conditions such as pulmonary disease.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Sprenger et al. (1994) Structure, genomic organization, and expressionof the human interleukin-8 receptor B gene. J Biol Chem 269:11065-11072.
Pease and Sabroe (2002) The role of interleukin-8 and its receptors in inflammatory lung disease: implications for therapy. Am J Respir Med 1:19-25.