MULTISCREEN™ β-ARRESTIN STABLE CELL LINES
HUMAN RECOMBINANT GPRC5A RECEPTOR
1 vial (2 x 106) frozen cells
Expression vector containing full-length human GPRC5A cDNA (GenBank Accession Number NM_003979.3) with FLAG tag sequence at N-terminus and ARRB2 cDNA (GenBank Accession Number NM_004313.3)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin, 250 µg/mL hygromycin
Stable for a minimum of 2 months in continuous culture
Background: GPRC5A (also known as retinoic induced GPR) is a member of the type three GPR class. This protein contains seven trans-membrane domains when located within the membrane. It appears to play a role in the interaction of retinoic acid and G protein signaling pathways. Since retinoic acid is important for cellular growth and differentiation, the receptor may play a role in embryonic development and cellular differentiation within epithelial cells.
Application: Functional assays
Figure 1. Basal arrestin recruitment levels, measured with MultiscreenTM β-Arrestin Assay Kit (Multispan MSBAK01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells
Zhou, H., Telonis, A., Jing, Y. et al. GPRC5A is a potential oncogene in pancreatic ductal adenocarcinoma cells that is upregulated by gemcitabine with help from HuR. Cell Death Dis 7, e2294 (2016).