MULTISCREEN™ β-ARRESTIN STABLE CELL LINES

HUMAN RECOMBINANT GPRC5A RECEPTOR

Product Information

Catalog Number:
CA1525

Lot Number:
CA1525-012320

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cellbanker 2

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human GPRC5A cDNA (GenBank Accession Number NM_003979.3) with FLAG tag sequence at N-terminus and ARRB2 cDNA (GenBank Accession Number NM_004313.3)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin, 250 µg/mL hygromycin

Stability:
Stable for a minimum of 2 months in continuous culture

Data Sheet

Background: GPRC5A (also known as retinoic induced GPR) is a member of the type three GPR class. This protein contains seven trans-membrane domains when located within the membrane. It appears to play a role in the interaction of retinoic acid and G protein signaling pathways. Since retinoic acid is important for cellular growth and differentiation, the receptor may play a role in embryonic development and cellular differentiation within epithelial cells.

Application: Functional assays

Figure 1. Basal arrestin recruitment levels, measured with MultiscreenTM β-Arrestin Assay Kit (Multispan MSBAK01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells

References:

Zhou, H., Telonis, A., Jing, Y. et al. GPRC5A is a potential oncogene in pancreatic ductal adenocarcinoma cells that is upregulated by gemcitabine with help from HuR. Cell Death Dis 7, e2294 (2016).

Zhou H, Rigoutsos I. The emerging roles of GPRC5A in diseases. Oncoscience. 2014;1(12):765–776. Published 2014 Nov 25. doi:10.18632/oncoscience.104

FOR RESEARCH USE ONLY.
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