MULTISCREEN™ β-ARRESTIN STABLE CELL LINES

HUMAN RECOMBINANT GIP RECEPTOR

Product Information

Catalog Number:
CA1290

Lot Number:
CA1290-071519

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cellbanker 2

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human GIPR cDNA (GenBank accession number NM_000164) with FLAG tag sequence at N-terminus and ARRB2 cDNA (GenBank Accession Number NM_004313.3)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin, 250 μg/mL hygromycin

Stability:
In progress

Data Sheet

Background: GIP (gastric inhibitory polypeptide) is released from the gastrointestinal tract, stimulates insulin secretion from pancreatic beta-cells, and plays a crucial role in the regulation of insulin secretion. Its receptor GIPR is expressed in the pancreas, stomach, small intestine, adipose tissue, adrenal cortex, pituitary, heart, testis, endothelial cells, bone, trachea, spleen, thymus, lung, kidney, thyroid, and several regions in the CNS. GIPR may have therapeutic potential in the treatment of type 2 diabetes and obesity.

Application: Functional assays

Figure 1. Dose-dependent stimulation from arrestin recruitment upon treatment with ligand, measured with MultiscreenTM β-Arrestin Assay Kit (Multispan MSBAK01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Irwin et al. (2009) Therapeutic potential for GIP receptor agonists and antagonists. Best Pract Res Clin Endocrinol Metab 23:499-512.

Yamada et al. (1995) Human gastric ingibitory polypeptide receptor: cloning of the gene (GIPR) and cDNA. Genomics 29:773-776.

FOR RESEARCH USE ONLY.
All rights reserved. No part of this document may be reproduced in any form without prior permission in writing.

26219 Eden Landing Road Hayward, CA 94545 | (510) 887-0817 | info@multispaninc.com