MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT CB1 MUTANT B RECEPTOR

Product Information

Catalog Number:
C1513-1

Lot Number:
C1513-1- 090717

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cell Banker 2 (Amsbio 11891)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human CB1B cDNA (GenBank Accession Number: NM_033181.3) with FLAG tag sequence at N-terminus.

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: Cannabinoid Receptor 1, CNR1 also known as CB1, is primarily involved in cannabinoid induced CNS effects. It acts by inhibiting intracellular adenylate cyclase activity and could be a receptor for anandamide.CB1 receptor is found in both central and peripheral tissues an recently, this receptor is also emerging as a potential therapeutic target for obesity-related diseases. It has two Isoforms CB1a and CB1b, CB1b is formed by internal deletion of 33 amino acids and preferentially expressed in B cells and Hepatocyte of Liver and is a more powerful modulator of adenyl cyclase activity.

Application: Functional assays

Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

González-Mariscal, I. et al.(2016) Human CB1 Receptor Isoforms, present in Hepatocytes and β-cells, are Involved in Regulating Metabolism. Sci. Rep. 6, 33302; doi: 10.1038/srep33302

Mendizabal, V. E. and E. Adler-Graschinsky (2003). “Cannabinoid system as a potential target for drug development in the treatment of cardiovascular disease.” Curr Vasc Pharmacol 1 (3): 301-13

Rinaldi-Carmona et al.. (1995) “Characterization of Two Cloned Human CB1 Cannabinoid Receptor Isoforms” JPET(278): 871-875

FOR RESEARCH USE ONLY.
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