MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT TP RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human TBXA2R cDNA (GenBank Accession Number NM_001060.4) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: The human TP (thromboxane A2) receptor is a very potent stimulator of platelet aggregation and a constrictor of vascular and respiratory smooth muscles. It has been shown to be a mediator in diseases such as myocardial infarction, stroke and bronchial asthma. TP receptors can be found on platelets, as well as macrophages, monocytes, vascular endothelial cells, and smooth muscle cells. TP receptor antagonists may also play a role in the treatment of atherothrombosis and stroke prevention.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent accumulation of intracellular IP1 upon treatment with ligand, measured with IP-one Tb kit (Cisbio 62IPAPEC). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Chamorro (2009) TP receptor antagonism: a new concept in atherothrombosis and stroke prevention. Cerebrovasc Dis 27 Suppl 3:20-27.
Hirata et al. (1991) Cloning and expression of cDNA for a human thromboxane receptor. Nature 349:617-20.
Nusing et al. (1993) Characterization and chromosomal mapping of the human thromboxane A2 receptor gene. J Biol Chem 268:25253-25259.