MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT NPFF1 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human NPFF1 cDNA (GenBank Accession Number NM_022146.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: Neuropeptide FF receptor 1 (NPFF1) acts as a modulator of endogenous opioid function. NPFF1 is found across the central nervous system. It is widely expressed in the hypothalamus and limbic system. Recent studies performed on NPPF1 receptors in mice implicate the receptor as an important regulator of body temperature.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.
Mollereau, Catherine et al. “Agonist and Antagonist Activities on Human NPFF2 Receptors of the NPY Ligands GR231118 and BIBP3226.” British Journal of Pharmacology 133.1 (2001): 1– 4. PMC. Web. 5 Mar. 2015.