MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT NOP (OPRL1) RECEPTOR

Product Information

Catalog Number:
C1354-1

Lot Number:
C1354-1-051209

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human OPRL1 cDNA (GenBank Accession Number NM_000913.3)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin

Stability:
Stable after minimum two months continuous growth

Data Sheet

Background: NOP, ORL1 or OPRL1 (opioid receptor-like 1 receptor) is also known as nociceptin receptor, orphanin FQ receptor and KOR-3 (kappa 3-related opioid receptor). It is a receptor for the neuropeptide nociceptin/orphanin FQ and has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. In vivo experiments have demonstrated that nociceptin modulates a variety of biological functions including nociception, food intake, memory processes, cardiovascular and renal functions, spontaneous locomotor activity, gastrointestinal motility, anxiety and neurotransmitter release at peripheral and central sites.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface as detected with an anti-ORL1 antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Calo et al. (2000) Pharmacology of nociceptin and its receptor: a novel therapeutic target. Br J Pharmacol 129:1261-1283.

Ciccocioppo et al. (2003) The nociceptin/orphanin FQ/NOP receptor system as a target for treatment of alcohol abuse: a review of recent work in alcoholpreferring rats. Physiol Behav 79:121-128.

FOR RESEARCH USE ONLY.
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