MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT OT RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length Human OXTR cDNA (GenBank Accession Number NM_000619.3) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DME/F12, 10% FBS, 10 µg/mL puromycin
Stable in culture for minimum of two months
Background: Oxytocin (OT) is a member of the neurohypophyseal hormone family. OT receptors are found in uterine smooth muscle, myoepithelial cells in the mammary gland, and in the pituitary. OT stimulates contraction of uterine smooth muscle during labor and stimulates milk secretion in response to suckling. It has been postulated that OT may also facilitate social and bonding behaviors related to the reproduction and care of offspring. Further studies of the OT receptor are essential in gaining a better understanding of the mechanism and in vivo regulation of uterine function.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent accumulation of intracellular IP1 upon treatment with ligand, measured with IP-one Tb kit (Cisbio 62IPAPEC). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Gimpl and Fahrenholz (2001) The oxytocin receptor system: structure, function, and regulation. Physiol Rev 81:629-683.
Ivell et al. (2001) The structure and regulation of the oxytocin receptor. Exp Physiol 86:289-296.
Shojo and Kaneko (2000) Characterization and expression of oxytocin and the oxytocin receptor. Mol Genet Metab 71:552-558.