MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT GHRH RECEPTOR

Product Information

Catalog Number:
C1291

Lot Number:
C1291-051506

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human GHRHR cDNA (GenBank Accession Number NM_000823) with FLAG tag sequence at N-terminus.

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable after minimum of two months continuous growth

Data Sheet

Background: Growth hormone-releasing hormone (GHRH), acting through the GHRH receptor GHRHR, plays a pivotal role in the regulation of GH synthesis and secretion in the pituitary. The isolated cDNA of the GHRHR predicted a 423-amino acid protein that has 7 putative transmembrane domains, characteristic of G protein- coupled receptors. It is a member of the secretin family of G protein-coupled receptors and has 47%, 42%, 35%, and 28% identity with receptors for vasoactive intestinal peptide, secretin, calcitonin and parathyroid hormone, respectively.

Application: Functional assays

Figure 1. Dose-dependent accumulation of intracellular cAMP upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Gaylinn et al. (1993) Molecular cloning and expression of a human anterior pituitary receptor for growth hormone-releasing hormone. Molec. Endocr 7:77-84.

DeAlmeida and Mayo (1998) Identification and binding domains of the growth hormone-releasing hormone receptor by analysis of mutant and chimeric receptor proteins. Molec Endocr 12:750-765.

FOR RESEARCH USE ONLY.
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