MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT Y5 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length Human NPY5R cDNA (GenBank Accession NM_006174) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: NPY5R encondes 456-amino acid protein with 7 putative transmembrane domains. It has shown to be expressed in the brain and hypothalamus. Y5 receptors’ pharmacological profile correlates it with peptides known to elicit a feeding response. It has been suggested to have effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Gerald et al. (1996) A receptor subtype involved in neuropeptide-Y induced food intake. Nature 382:168-171.
Hu et al. (1996) Identification of a novel hypothalamic neuropeptide Y receptor associated with feeding behavior. J Biol Chem 217:26315-26319.