MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT MRGX2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human MRGX2 cDNA (GenBank Accession Number NM_054030)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 200 µg/mL hygromycin, 0.625 µg/mL puromycin
Stable after minimum of two months continuous growth
Background: MRGX2 (MAS-related GPR member X2) is a receptor for cortistatin. It is probably involved in the function of nociceptive neurons and regulation of nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14, a high potency agonist at the receptor, has biological functions including sleep regulation, locomotor activity and cortical function.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Robas et al. (2003) MrgX2 is a high potency cortistatin receptor expressed in dorsal root ganglion. J Biol Chem 278:44400-44404.
Kamohara et al. (2005) Identification of MrgX2 as a human G-protein-coupled receptor for proadrenomedullin N-terminal peptides. Biochem Biophys Res Commun 330:1146-1152.