MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT MRGX2 RECEPTOR

Product Information

Catalog Number:
C1257

Lot Number:
C1257-073005

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T Gαqi5

Transfection:
Full-length human MRGX2 cDNA (GenBank Accession Number NM_054030)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 200 µg/mL hygromycin, 0.625 µg/mL puromycin

Stability:
Stable after minimum of two months continuous growth

Data Sheet

Background: MRGX2 (MAS-related GPR member X2) is a receptor for cortistatin. It is probably involved in the function of nociceptive neurons and regulation of nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14, a high potency agonist at the receptor, has biological functions including sleep regulation, locomotor activity and cortical function.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Robas et al. (2003) MrgX2 is a high potency cortistatin receptor expressed in dorsal root ganglion. J Biol Chem 278:44400-44404.

Kamohara et al. (2005) Identification of MrgX2 as a human G-protein-coupled receptor for proadrenomedullin N-terminal peptides. Biochem Biophys Res Commun 330:1146-1152.

FOR RESEARCH USE ONLY.
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