MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT ALX RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human FPR2 cDNA (GenBank Accession Number NM_001462.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin
Stable after minimum two months of continuous growth
Background: The lipoxin A4 receptor ALX is also known as formyl peptide receptor- like 1 (FPRL1) or formyl peptide receptor 2 (FPR2). It shares 69% amino acid identity with FPR but displays low affinity for bacterial peptide N-formyl-methionyl- leucyl-phenylalanine (fMLF). ALX is highly expressed on neutrophils and monocytes and mediates cell chemotaxis. By interacting with a variety of exogenous and host- derived agonists, ALX may have important implications in the pathogenesis of human diseases such as HIV, Alzheimer’s disease (AD), amyloidosis and prion diseases.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Le et al. (2002) Formyl-peptide receptors revisited. Trends Immunol 23:541-548. Migeotte et al. (2005) Identification and characterization of an endogenous
chemotactic ligand specific for FPRL2. J Exp Med 201:83-93.