MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT EP2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human EP2 cDNA (GenBank Accession Number NM_000956.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after minimum two months continuous growth
Background: The human prostaglandin E2 (PGE2) receptor EP2 (PTGER2) is abundantly expressed in various tissues including the corneal epithelium of the eye, spinal cord, forebrain, articular cartilage, and kidney. EP2 plays important roles in bronchodilation, dilation of arterioles and venules, blood pressure regulation, smooth muscle relaxation, and bone formation. Modification of PGE2-EP2 receptor signaling may provide a new therapeutic strategy for renal regulation and blood pressure illnesses, as well as bone disease such as osteoarthritis.
Application: Functional assays
Figure 1. Dose-dependent accumulation of intracellular cAMP upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Morath et al. (1999) Immunolocalization of the four prostaglandin E2 receptor proteins EP1, EP2, EP3, and EP4 in human kidney. J Am Soc Nephrol 10:1851- 1860.
Zhang et al. (2000) Characterization of murine vasopressor and vasodepressor prostaglandin E(2) receptors. Hypertension 35:1129-1134.
Li X et al. (2009) Prostaglandin E(2) and its cognate EP receptors control human adult articular cartilage homeostasis and are linked to the pathophysiology of osteoarthritis. Arthritis Rheum 60:513-523.