MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT MGLUR5 RECEPTOR
1 vial (4 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human GRM5 cDNA (NM_000842.1) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM with GlutaMAX (Gibco 10566), 10% FBS (dialyzed), 2 mM sodium pyruvate, 1 μg/mL puromycin
Stable after two months of continuous growth
Background: The metabotropic glutamate receptors (mGluRs) are divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacological properties. Group II and group III mGluRs are linked to inhibition of the cyclic AMP cascade, but differ in their agonist selectivity. mGluR5 is a member of Group I. It mainly expresses the brain although its precise role in various cortical functions remains unclear.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Romano et al. (1995) Distribution of metabotropic glutamate receptor mGluR5 immunoreactivity in rat brain. J Comp Neruol 355:455-469.
She et al. (2009) Roles of mGluR5 in synaptic function and plasticity of the mouse thalamocortical pathway. Eur J Neurosci 29:1379-1396.