MULTISCREEN™ STABLE CELL LINES
RAT RECOMBINANT TSH RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length Rat Tshr cDNA (GenBank Accession Number NM_012888.1)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 100µg/mL zeocin
Stable after minimum of two months continuous growth
Background: The thyroid stimulating hormone receptor (TSHR) is expressed in the membrane of thyroid follicular cells. It is involved in regulating thyrocyte cell growth and function. TSHR is also the target autoantigen in thyroid autoimmune diseases. Autoantibodies to TSHR that act as agonists are responsible for the hyperthyroidism of Graves’ Disease. Another class of autoantibodies that block the binding of TSH to TSHR may mediate the hypothyroidism associated with Hashimoto’s thyroiditis, primary myxoedema, and neonatal hypothyroidism.
Application: Functional assays
Figure Legend. Dose-dependent stimulation of intercellular calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Morgenthaler et al. (1999) Direct binding of thyrotropin receptor autoantibody to in vitro translated thyrotropin receptor: a comparison to radioreceptor assay and thyroid stimulating bioassay. Thyroid 9:466-475.
Davies et al. (2005) Thyrotropin receptor-associated diseases: from adenomata to Graves disease. J Clin Invest 115:1972-1983.
Ando, T and Davies TF (2005) Monoclonal antibodies to the thyrotropin receptor. Clin Dev Immunol 12:137-43.